Imiti yimiti muri fermentation ya hydroxypropyl methylcellulose

Hydroxypropyl methylcellulose (HPMC)ni ifumbire mvaruganda polymer ikunze gukoreshwa mubikorwa byinganda nubuvuzi, kandi ifite indangagaciro zitandukanye zikoreshwa, nko mubisohoka bigenzurwa nibiyobyabwenge, gutunganya ibiryo nibikoresho byubaka. Imyitwarire yimiti mubikorwa byayo byo gusembura ifitanye isano cyane no kwangirika no guhindura selile hamwe nibikorwa bya metabolike ya mikorobe. Kugirango twumve neza imiterere yimiti ya HPMC mugikorwa cya fermentation, dukeneye mbere na mbere gusobanukirwa imiterere yibanze hamwe nuburyo bwo kwangirika kwa selile.

1. Imiterere yibanze nimiterere ya hydroxypropyl methylcellulose

HPMC ni inkomoko yabonetse muguhindura imiti ya selile isanzwe (Cellulose). Umugongo wumunyururu wa molekile ni molekile ya glucose (C6H12O6) ihujwe na β-1,4 glycosidic. Cellulose ubwayo iragoye gushonga mumazi, ariko mugutangiza methyl (-OCH3) na hydroxypropyl (-C3H7OH), imbaraga zayo zamazi zirashobora kunozwa cyane kugirango habeho polymer zishonga. Uburyo bwo guhindura HPMC muri rusange burimo reaction ya selile hamwe na methyl chloride (CH3Cl) na alcool ya propylene (C3H6O) mubihe bya alkaline, kandi ibicuruzwa bivamo bifite hydrophilicity na solubilité.

2. Imyitwarire yimiti mugihe cya fermentation

Uburyo bwa fermentation ya HPMC mubisanzwe biterwa nigikorwa cya mikorobe, ikoresha HPMC nkisoko ya karubone nisoko yintungamubiri. Inzira ya fermentation ya HPMC ikubiyemo ibyiciro byingenzi bikurikira:

2.1. Gutesha agaciro HPMC

Cellulose ubwayo igizwe na glucose ihujwe, kandi HPMC izangirika na mikorobe mugihe cya fermentation, ibanza kubora isukari ntoya ikoreshwa (nka glucose, xylose, nibindi). Ubu buryo busanzwe bukubiyemo ibikorwa bya selile nyinshi zangiza. Ibintu nyamukuru bitesha agaciro harimo:

Cellulose hydrolysis reaction: Bond-1,4 ya glycosideque muri molekile ya selile izasenywa na hydrolase ya selile (nka selile, endocellulase), itanga iminyururu ngufi yisukari (nka oligosaccharide, disaccharide, nibindi). Isukari izarushaho guhindagurika no gukoreshwa na mikorobe.

Hydrolysis no gutesha agaciro HPMC: Ibikoresho bya methyl na hydroxypropyl muri molekile ya HPMC bizakurwaho igice na hydrolysis. Uburyo bwihariye bwibikorwa bya hydrolysis ntiburasobanuka neza, ariko dushobora kuvuga ko mugihe cya fermentation, reaction ya hydrolysis iterwa na enzymes zasohowe na mikorobe (nka hydroxyl esterase). Iyi nzira iganisha kumeneka ya HPMC iminyururu no gukuraho amatsinda akora, amaherezo agakora molekile ntoya.

2.2. Microbial metabolic reaction

HPMC imaze kwangirika muri molekile ntoya yisukari, mikorobe irashobora guhindura isukari imbaraga binyuze mumikorere ya enzymatique. By'umwihariko, mikorobe ibora glucose muri Ethanol, aside ya lactique cyangwa izindi metabolite binyuze mu nzira ya fermentation. Ibinyabuzima bitandukanye bishobora guhinduranya ibicuruzwa bitesha agaciro HPMC binyuze munzira zitandukanye. Inzira zisanzwe zo guhinduranya zirimo:

Inzira ya Glycolysis: Glucose ibora muri pyruvate na enzymes hanyuma igahinduka imbaraga (ATP) na metabolite (nka acide lactique, Ethanol, nibindi).

Ibibyara umusaruro wa fermentation: Mugihe cya anaerobic cyangwa hypoxic, mikorobe ihindura glucose cyangwa ibicuruzwa byayo byangirika muri acide kama nka Ethanol, acide lactique, acide acetike, nibindi binyuze mumihanda ya fermentation, ikoreshwa cyane mubikorwa bitandukanye byinganda.

2.3. Redox reaction

Mugihe cyo gusembura HPMC, mikorobe zimwe zishobora kurushaho guhindura ibicuruzwa hagati binyuze muri redox reaction. Kurugero, inzira yo gukora Ethanol iherekejwe na redox reaction, glucose ihindurwamo okiside kugirango itange pyruvate, hanyuma pyruvate ihindurwamo Ethanol binyuze mubigabanya. Izi reaction ningirakamaro mugukomeza metabolike yingirabuzimafatizo.

3. Kugenzura ibintu mubikorwa bya fermentation

Mugihe cya fermentation ya HPMC, ibintu bidukikije bigira uruhare runini mubitekerezo byimiti. Kurugero, pH, ubushyuhe, ibirimo ogisijeni yashonze, intungamubiri zintungamubiri, nibindi bizagira ingaruka kumuvuduko wa metabolike ya mikorobe nubwoko bwibicuruzwa. Cyane cyane ubushyuhe na pH, ibikorwa byimisemburo ya mikorobe birashobora gutandukana cyane mubihe bitandukanye nubushyuhe bwa pH, bityo rero birakenewe kugenzura neza imiterere ya fermentation kugirango harebwe iyangirika rya HPMC niterambere ryimikorere ya metabolike ya mikorobe.

Inzira ya fermentation yaHPMCikubiyemo imiti igoye, harimo hydrolysis ya selile, iyangirika rya HPMC, metabolisme yisukari, hamwe no kubyara fermentation. Gusobanukirwa nibi bitekerezo ntabwo bifasha gusa kunonosora inzira ya fermentation ya HPMC, ahubwo binatanga inkunga yibyerekeranye nibikorwa byinganda. Hamwe n’ubushakashatsi bwimbitse, uburyo bunoze kandi bwubukungu bwa fermentation burashobora gutezwa imbere mugihe kizaza kugirango habeho kunoza imikorere ya HPMC numusaruro wibicuruzwa, no guteza imbere ikoreshwa rya HPMC mubinyabuzima, kurengera ibidukikije nizindi nzego.